BIOTEX-biosensing textiles for personalised healthcare management.

Textile-based sensors offer an unobtrusive method of continually monitoring physiological parameters during daily activities. Chemical analysis of body fluids, noninvasively, is a novel and exciting area of personalized wearable healthcare systems. BIOTEX was an EU-funded project that aimed to develop textile sensors to measure physiological parameters and the chemical composition of body fluids, with a particular interest in sweat. A wearable sensing system has been developed that integrates a textile-based fluid handling system for sample collection and transport with a number of sensors including sodium, conductivity, and pH sensors. Sensors for sweat rate, ECG, respiration, and blood oxygenation were also developed. For the first time, it has been possible to monitor a number of physiological parameters together with sweat composition in real time. This has been carried out via a network of wearable sensors distributed around the body of a subject user. This has huge implications for the field of sports and human performance and opens a whole new field of research in the clinical setting

ENJEUX CLINIQUES ET DE SANTE PUBLIQUE D'UNE PRISE EN CHARGE GLOBALE MEDICALE ET SOCIALE DES PATIENTS (EVALUATION DE 400 DOSSIERS DE LA POLICLINIQUE DE L'HOPITAL DE NANTERRE. PREMIERE : PARTIE : EVALUATION DES BESOINS MEDICO-SOCIAUX DE SOINS ET DE PREVENTION, ET DES REPONSES NECESSAIRES)

PARIS7-Xavier Bichat (751182101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

The use of ultrasound biomicroscopy for embryo injections

Ultrasound biomicroscopy (UBM) is based on the use of > 25 MHz probes enabling high resolution (<100μ) in vivo visualisation of structures up to 1cm deep. It is currently used in ophthalmology and biomedical research (rodents). Ultrasound-guidance is used to improve the accuracy of needle placement for in vivo injections. In developmental biology, the in vitro and in vivo injection of cells, tracer dyes and adenoviral or retroviral vectors into embryos are powerful approaches for studying developmental processes. After implantation, however, the conceptus is encased in the uterus and in vivo injections into the conceptus become a technical challenge when performed in a "blind" manner. In utero microinjection under UBM guidance was shown to be a useful tool for exploring the developmental consequences of altering gene expression and for studying cell lineage or migration during early embryonic and placental development in mice. For example ectoplacental cone region, amniotic cavity and exocoelomic cavity were accurately targeted at E6.5 and E7.5 and a fluorescent bead suspension was injected to study early placental and embryonic development. We have adapted this technique to the rabbit, targeting embryos in vivo at the peri-implantation stage (day 6 to 7.5). As a proof of concept, we performed an experiment on 4 female rabbits. Females were time-mated. On day 6.75, they were anaesthetized and a laparotomy was performed. Uterine horns were exteriorised. Two embryos per doe were injected with sterile saline. Animals were euthanized on Day14. We obtained 2 well-developed live conceptuses after UBM guided embryo injection. In conclusion, UBM makes it possible to puncture rabbit embryos at the peri-implantation stage without compromising normal development. This technique is relevant for any injection or sampling into the embryo with subsequent study of development